5a5 mouse mab Search Results


5a5 mouse mab  (Developmental Studies Hybridoma Bank)
95
Developmental Studies Hybridoma Bank 5a5 mouse mab
5a5 Mouse Mab, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5a5 mouse mab - by Bioz Stars, 2026-07
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beta-actin antibody  (NSJ Bioreagents)
92
NSJ Bioreagents beta-actin antibody
Beta Actin Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibody against nkx2 2  (Developmental Studies Hybridoma Bank)
96
Developmental Studies Hybridoma Bank monoclonal antibody against nkx2 2
Monoclonal Antibody Against Nkx2 2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal antibody against nkx2 2 - by Bioz Stars, 2026-07
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mouse anti lmx1b monoclonal antibody  (Developmental Studies Hybridoma Bank)
90
Developmental Studies Hybridoma Bank mouse anti lmx1b monoclonal antibody
Mouse Anti Lmx1b Monoclonal Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mouse anti lmx1b monoclonal antibody - by Bioz Stars, 2026-07
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monoclonal mouse anti hsp 70 antibody  (Danaher Inc)
99
Danaher Inc monoclonal mouse anti hsp 70 antibody
Monoclonal Mouse Anti Hsp 70 Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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monoclonal mouse anti hsp 70 antibody - by Bioz Stars, 2026-07
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anti nkx2 2 monoclonal  (Developmental Studies Hybridoma Bank)
93
Developmental Studies Hybridoma Bank anti nkx2 2 monoclonal
Anti Nkx2 2 Monoclonal, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti nkx2 2 monoclonal - by Bioz Stars, 2026-07
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anti nkx2 2  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology anti nkx2 2
The identity of the CR2-GFP+ cells was examined in the transgenic spinal cord with immunofluorescence staining. GFP+ cells were stained with Notch1 ( a ) and neural stem cell marker Sox2 ( b ) at E9.5. Histograms show the quantification of the co-labeled GFP+ cells. Staining with the domain specific markers <t>Nkx2.2,</t> Pax6 and Pax7 ( c ) showed that at E9.5 GFP+ cells were localized to the dorsal progenitor layer 1–3 (dP1–3), motoneuron progenitor layer (pMN), and ventral progenitor layer 3 (pV3). Schematic drawing depicts the distribution of GFP+ cells in the E9.5 spinal cord. Staining of a post-mitotic neuronal marker NeuN showed little co-labeling with GFP from E9.5 to E15.5 ( d ), suggesting that CR2-GFP is not expressed in the differentiated neurons. However, the GFP+ progenitor cells differentiated into NeuN+ cells as suggested by cell-fate-tracing with anti-GFP antibody ( e ). Cell counting suggests a dramatic increase of GFP+/NeuN+ cells with anti-GFP tracing ( f ). Arrows indicate the co-labeled cells ( a,b,d,e ) or GFP+ cells ( c ). D, dorsal; V, ventral. Scale bars = 100 μm. T-test, ***p-value < 0.005; n ≥ 3.
Anti Nkx2 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/5a5+mouse+mab/pmc05141430-19-42-23?v=Santa+Cruz+Biotechnology
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mouse monoclonal anti ssea1 antibody  (Developmental Studies Hybridoma Bank)
96
Developmental Studies Hybridoma Bank mouse monoclonal anti ssea1 antibody
The identity of the CR2-GFP+ cells was examined in the transgenic spinal cord with immunofluorescence staining. GFP+ cells were stained with Notch1 ( a ) and neural stem cell marker Sox2 ( b ) at E9.5. Histograms show the quantification of the co-labeled GFP+ cells. Staining with the domain specific markers <t>Nkx2.2,</t> Pax6 and Pax7 ( c ) showed that at E9.5 GFP+ cells were localized to the dorsal progenitor layer 1–3 (dP1–3), motoneuron progenitor layer (pMN), and ventral progenitor layer 3 (pV3). Schematic drawing depicts the distribution of GFP+ cells in the E9.5 spinal cord. Staining of a post-mitotic neuronal marker NeuN showed little co-labeling with GFP from E9.5 to E15.5 ( d ), suggesting that CR2-GFP is not expressed in the differentiated neurons. However, the GFP+ progenitor cells differentiated into NeuN+ cells as suggested by cell-fate-tracing with anti-GFP antibody ( e ). Cell counting suggests a dramatic increase of GFP+/NeuN+ cells with anti-GFP tracing ( f ). Arrows indicate the co-labeled cells ( a,b,d,e ) or GFP+ cells ( c ). D, dorsal; V, ventral. Scale bars = 100 μm. T-test, ***p-value < 0.005; n ≥ 3.
Mouse Monoclonal Anti Ssea1 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
mouse monoclonal anti ssea1 antibody - by Bioz Stars, 2026-07
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mab 5a5  (Developmental Studies Hybridoma Bank)
94
Developmental Studies Hybridoma Bank mab 5a5
The identity of the CR2-GFP+ cells was examined in the transgenic spinal cord with immunofluorescence staining. GFP+ cells were stained with Notch1 ( a ) and neural stem cell marker Sox2 ( b ) at E9.5. Histograms show the quantification of the co-labeled GFP+ cells. Staining with the domain specific markers <t>Nkx2.2,</t> Pax6 and Pax7 ( c ) showed that at E9.5 GFP+ cells were localized to the dorsal progenitor layer 1–3 (dP1–3), motoneuron progenitor layer (pMN), and ventral progenitor layer 3 (pV3). Schematic drawing depicts the distribution of GFP+ cells in the E9.5 spinal cord. Staining of a post-mitotic neuronal marker NeuN showed little co-labeling with GFP from E9.5 to E15.5 ( d ), suggesting that CR2-GFP is not expressed in the differentiated neurons. However, the GFP+ progenitor cells differentiated into NeuN+ cells as suggested by cell-fate-tracing with anti-GFP antibody ( e ). Cell counting suggests a dramatic increase of GFP+/NeuN+ cells with anti-GFP tracing ( f ). Arrows indicate the co-labeled cells ( a,b,d,e ) or GFP+ cells ( c ). D, dorsal; V, ventral. Scale bars = 100 μm. T-test, ***p-value < 0.005; n ≥ 3.
Mab 5a5, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
mab 5a5 - by Bioz Stars, 2026-07
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actin antibody  (NSJ Bioreagents)
99
NSJ Bioreagents actin antibody
The identity of the CR2-GFP+ cells was examined in the transgenic spinal cord with immunofluorescence staining. GFP+ cells were stained with Notch1 ( a ) and neural stem cell marker Sox2 ( b ) at E9.5. Histograms show the quantification of the co-labeled GFP+ cells. Staining with the domain specific markers <t>Nkx2.2,</t> Pax6 and Pax7 ( c ) showed that at E9.5 GFP+ cells were localized to the dorsal progenitor layer 1–3 (dP1–3), motoneuron progenitor layer (pMN), and ventral progenitor layer 3 (pV3). Schematic drawing depicts the distribution of GFP+ cells in the E9.5 spinal cord. Staining of a post-mitotic neuronal marker NeuN showed little co-labeling with GFP from E9.5 to E15.5 ( d ), suggesting that CR2-GFP is not expressed in the differentiated neurons. However, the GFP+ progenitor cells differentiated into NeuN+ cells as suggested by cell-fate-tracing with anti-GFP antibody ( e ). Cell counting suggests a dramatic increase of GFP+/NeuN+ cells with anti-GFP tracing ( f ). Arrows indicate the co-labeled cells ( a,b,d,e ) or GFP+ cells ( c ). D, dorsal; V, ventral. Scale bars = 100 μm. T-test, ***p-value < 0.005; n ≥ 3.
Actin Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
actin antibody - by Bioz Stars, 2026-07
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mouse anti-actin mab  (US Biological Life Sciences)
90
US Biological Life Sciences mouse anti-actin mab
The identity of the CR2-GFP+ cells was examined in the transgenic spinal cord with immunofluorescence staining. GFP+ cells were stained with Notch1 ( a ) and neural stem cell marker Sox2 ( b ) at E9.5. Histograms show the quantification of the co-labeled GFP+ cells. Staining with the domain specific markers <t>Nkx2.2,</t> Pax6 and Pax7 ( c ) showed that at E9.5 GFP+ cells were localized to the dorsal progenitor layer 1–3 (dP1–3), motoneuron progenitor layer (pMN), and ventral progenitor layer 3 (pV3). Schematic drawing depicts the distribution of GFP+ cells in the E9.5 spinal cord. Staining of a post-mitotic neuronal marker NeuN showed little co-labeling with GFP from E9.5 to E15.5 ( d ), suggesting that CR2-GFP is not expressed in the differentiated neurons. However, the GFP+ progenitor cells differentiated into NeuN+ cells as suggested by cell-fate-tracing with anti-GFP antibody ( e ). Cell counting suggests a dramatic increase of GFP+/NeuN+ cells with anti-GFP tracing ( f ). Arrows indicate the co-labeled cells ( a,b,d,e ) or GFP+ cells ( c ). D, dorsal; V, ventral. Scale bars = 100 μm. T-test, ***p-value < 0.005; n ≥ 3.
Mouse Anti Actin Mab, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
mouse anti-actin mab - by Bioz Stars, 2026-07
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anti-hwasp monoclonal antibody 5a5  (Becton Dickinson)
90
Becton Dickinson anti-hwasp monoclonal antibody 5a5
The identity of the CR2-GFP+ cells was examined in the transgenic spinal cord with immunofluorescence staining. GFP+ cells were stained with Notch1 ( a ) and neural stem cell marker Sox2 ( b ) at E9.5. Histograms show the quantification of the co-labeled GFP+ cells. Staining with the domain specific markers <t>Nkx2.2,</t> Pax6 and Pax7 ( c ) showed that at E9.5 GFP+ cells were localized to the dorsal progenitor layer 1–3 (dP1–3), motoneuron progenitor layer (pMN), and ventral progenitor layer 3 (pV3). Schematic drawing depicts the distribution of GFP+ cells in the E9.5 spinal cord. Staining of a post-mitotic neuronal marker NeuN showed little co-labeling with GFP from E9.5 to E15.5 ( d ), suggesting that CR2-GFP is not expressed in the differentiated neurons. However, the GFP+ progenitor cells differentiated into NeuN+ cells as suggested by cell-fate-tracing with anti-GFP antibody ( e ). Cell counting suggests a dramatic increase of GFP+/NeuN+ cells with anti-GFP tracing ( f ). Arrows indicate the co-labeled cells ( a,b,d,e ) or GFP+ cells ( c ). D, dorsal; V, ventral. Scale bars = 100 μm. T-test, ***p-value < 0.005; n ≥ 3.
Anti Hwasp Monoclonal Antibody 5a5, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-hwasp monoclonal antibody 5a5 - by Bioz Stars, 2026-07
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Image Search Results


The identity of the CR2-GFP+ cells was examined in the transgenic spinal cord with immunofluorescence staining. GFP+ cells were stained with Notch1 ( a ) and neural stem cell marker Sox2 ( b ) at E9.5. Histograms show the quantification of the co-labeled GFP+ cells. Staining with the domain specific markers Nkx2.2, Pax6 and Pax7 ( c ) showed that at E9.5 GFP+ cells were localized to the dorsal progenitor layer 1–3 (dP1–3), motoneuron progenitor layer (pMN), and ventral progenitor layer 3 (pV3). Schematic drawing depicts the distribution of GFP+ cells in the E9.5 spinal cord. Staining of a post-mitotic neuronal marker NeuN showed little co-labeling with GFP from E9.5 to E15.5 ( d ), suggesting that CR2-GFP is not expressed in the differentiated neurons. However, the GFP+ progenitor cells differentiated into NeuN+ cells as suggested by cell-fate-tracing with anti-GFP antibody ( e ). Cell counting suggests a dramatic increase of GFP+/NeuN+ cells with anti-GFP tracing ( f ). Arrows indicate the co-labeled cells ( a,b,d,e ) or GFP+ cells ( c ). D, dorsal; V, ventral. Scale bars = 100 μm. T-test, ***p-value < 0.005; n ≥ 3.

Journal: Scientific Reports

Article Title: Transcriptional Regulation of Notch1 Expression by Nkx6.1 in Neural Stem/Progenitor Cells during Ventral Spinal Cord Development

doi: 10.1038/srep38665

Figure Lengend Snippet: The identity of the CR2-GFP+ cells was examined in the transgenic spinal cord with immunofluorescence staining. GFP+ cells were stained with Notch1 ( a ) and neural stem cell marker Sox2 ( b ) at E9.5. Histograms show the quantification of the co-labeled GFP+ cells. Staining with the domain specific markers Nkx2.2, Pax6 and Pax7 ( c ) showed that at E9.5 GFP+ cells were localized to the dorsal progenitor layer 1–3 (dP1–3), motoneuron progenitor layer (pMN), and ventral progenitor layer 3 (pV3). Schematic drawing depicts the distribution of GFP+ cells in the E9.5 spinal cord. Staining of a post-mitotic neuronal marker NeuN showed little co-labeling with GFP from E9.5 to E15.5 ( d ), suggesting that CR2-GFP is not expressed in the differentiated neurons. However, the GFP+ progenitor cells differentiated into NeuN+ cells as suggested by cell-fate-tracing with anti-GFP antibody ( e ). Cell counting suggests a dramatic increase of GFP+/NeuN+ cells with anti-GFP tracing ( f ). Arrows indicate the co-labeled cells ( a,b,d,e ) or GFP+ cells ( c ). D, dorsal; V, ventral. Scale bars = 100 μm. T-test, ***p-value < 0.005; n ≥ 3.

Article Snippet: The following primary antibodies were used: anti-Notch1 (rabbit, polyclonal, 1:100, 6014-R), anti-Gata2 (rabbit, polyclonal, 1:200, sc-9008x) and anti-Chx10 (goat, polyclonal, 1:300, sc-21692) from Santa Cruz Biotechnology, Inc.; anti-En1 (mouse, monoclonal, 1:1000, 4G11-c), anti-Evx1 (mouse, monoclonal, 1:10, 99.1–3A2), anti-Isl1 (mouse, monoclonal, 1:100, 39.4D5), anti-Nkx2.2 (mouse, monoclonal, 1:10, 74.5A5), anti-Nkx6.1 (mouse, monoclonal, 1:25, F55A12), anti-Pax7 (mouse, monoclonal, 1:25) and anti-Pax6 (mouse, monoclonal, 1:15) from Developmental Studies Hybridoma Bank (DSHB); anti-Brn3a (mouse, monoclonal, 1:100, MAB1585), anti-NeuN (mouse, monoclonal, 1:1000, MAB377) and anti-Sox2 (mouse, monoclonal, 1:500, MAB4343) from Millipore; anti-GFP (goat, polyclonal, 1:1000, AB5450) from Abcam; anti-GFP (rabbit, polyclonal, 1:500, a11122) from Invitrogen; anti-S100b (mouse, monoclonal, 1:1000, S2532) from Sigma; anti-Pax2 (rabbit, polyclonal, 1:250, 71–6000) from Zymed; anti-Olig2 (rabbit, polyclonal, 1:10000, gift from Dr. Charles Stiles at Harvard University).

Techniques: Transgenic Assay, Immunofluorescence, Staining, Marker, Labeling, Cell Counting